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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Oxyresveratrol Inhibits IL-1β-Induced Inflammation via Suppressing AKT and ERK1/2 Activation in Human Microglia, HMC3
doi: 10.3390/ijms21176054
Figure Lengend Snippet: ( A and D ) Western blot analysis for the inhibitory effects of OXY on the phosphorylation of AKT, p70S6K, and ERK1/2 in cells treated with various concentrations of OXY (10, 20, and 40 μM) for 4 h before stimulation with IL-1β for 20 min; ( B ) quantitative analysis for AKT phosphorylation at Ser473; ( C ) quantitative analysis for p70S6K phosphorylation; ( E ) quantitative analysis for ERK1/2 phosphorylation. Total AKT, total ERK1/2, and beta actin (β-actin) were detected and used as an internal control. Data are represented as mean ± SD of three independent experiments; # p < 0.05 (compared to the untreated group) or * p < 0.05 (compared to the IL-1β-treated group).
Article Snippet: After blocked with 5% skim milk in a mixture of tris-buffered saline and Tween 20 (TBS-T) (0.02 M Tris-HCl, pH 7.6, 0.0137 M NaCl, and 0.05% Tween 20) (all reagents from Sigma-Aldrich, Saint Louis, MO, USA) at RT for 1 h, membranes were incubated with an appropriate primary antibody (Cell Signaling Technology, Boston, MA, USA) at 4 °C for 24 h. Primary antibodies used in this study included an anti-NF-κB p65 (D14E12) antibody (catalog number 8242), a mouse anti-IκBα (L35A5) antibody (catalog number 4814), a phosphospecific rabbit anti-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (catalog number 4370), a mouse anti-p44/42 MAPK (Erk1/2) antibody (catalog number 9107), a phosphospecific mouse anti-p38 MAPK (Thr180/Tyr182) antibody (catalog number 9216), a rabbit anti-p38 MAPK (D13E1) antibody (catalog number 8690), a phosphospecific rabbit anti-SAPK/JNK (Thr183/Tyr185) antibody (catalog number 4668), a rabbit anti-SAPK/JNK antibody (catalog number 9252), a phosphospecific rabbit anti-AKT (Ser473) antibody (catalog number 4060), a mouse anti-AKT (40D4) antibody (catalog number 2920), a phosphospecific rabbit p70 S6 kinase (Thr389) antibody (catalog number 97596), or a
Techniques: Western Blot, Phospho-proteomics, Control